Hyaluronic acid treatment
Hyaluronic acid
The new treatment does not alter the roughness and it speeds up the osseointegration process
Biochemical modifications of Ti implant surfaces (BMTIS)
HYALURONIC ACID (HYHA)
Partial surface treatment with hyaluronic acid on the implant body. Cold plasma decontamination.
Biochemical methods of surface modification strive to use the current understanding of the biology and biochemistry of cellular function and differentiation. “Biochemical surface modification uses critical organic components of bone to affect tissue response”. The goal of biochemical modifications is to immobilize proteins, enzymes or peptides on devices surfaces to induce specific cell and tissue responses. Puleo da Nanci a (1999): understanding and controlling the bone-implant interface, biomaterials, 20:2311-2321.
MAG 52 X WD 11.5 mm EHT 20.00 kV Signal A CZ BSD
MAG 200 X WD 11.0 mm EHT 20.00 kV Signal A CZ BSD
MAG 1.50 K X WD 11.5 mm EHT 20.00 kV Signal A SE1
Hyaluronic acid and osseointegration
In 1904 Pfaundler hypothesized that calcium-binding was an important step during the calcification of the bone and that some unknown component of the bone was responsible; It was later discovered that GAGs play an important role and that HA enhances proliferation and growth of hydroxyapatite crystals; Iwata and Urist found that large amounts of HA were secreted when implants of decalcified bone remineralized as bone (Clin. Orthop. Rel. Res., 90, 236, 1973); In vitro studies using fetal calvarial cells and bone marrow osteogenic stem cells show that osteogenesis in vitro is significantly enhanced by HA 30-160 kDa, while high Mw HA (550-1300 kDa) shows weak inhibitory effect as compared to the control (G. W. Bernard et al. Redefining Hyaluronan, Abatangelo and Weigel Eds., Elsevier, 2000, pp. 215-231); 800 kDa HA added to bone marrow stromal cells cultured in vitro accelerates cell proliferation, increases ALP activity and osteocalcin gene expression. HA interacts with BMP-2 to generate direct and specific cellular effects (X. Zou et al. Biomaterials 25, 5375, 2004); 900 kDa HA has a positive effect on bone ingrowth in Titanium fibre mesh implant in rats (S. Itoh et al. J. Mat. Sci. Mat. Med., 12, 575, 2001); HA shows a positive effect in early bone consolidation in distraction osteogenesis (B. C. Cho et al., J. Craniofacial Surg., 13, 783, 2002).
Sa 0,50 µm
Overall mean value on a measuring area of 30×30 μm, cold plasma decontamination.
Sa 1,90 µm
Overall mean value on a measuring area of 30×30 μm, sand-blasting, double etching, cold plasma decontamination.
Hyaluronic acid and Ti implants
Results (cortical bone)
Descriptive studies: histomorphometry (n = 5)
BIC: the length of bone directly opposed to the implant without the presence of a fibrous membrane/the total length of the bone-implant interface x 100 Bone ingrowth: bone area between the screw and the line connecting the thread crests divided by the total screw thread area x 100.
Evaluation of newly-formed bone quality: microhardness of interfacial bone (n = 5)
Microhardness measurements were performed in the tangential direction to the interface with a Vickers indenter (four-sided pyramid with square base and an apex angle between opposite sides of 136°±15’) applied at a load of 0.05 kgf and dwell time of 5 sec.
Functional studies: mechanical tests (n = 10)
The push-out test was carried out by placing the femoral segments on a support jig (∅ 3.5 mm) using a MTS apparatus (Sintech-1/M, MTS Adamel Lhomargy, Ivry sur Seine, France). A force was applied to the implant from the medullar side at a constant crosshead speed of 2 mm/min, pushing it out from its bony bed.
Results (trabecular bone)
Histomorphometric results for uncoated and coated screws IN TRABECULAR BONE at 4 weeks (n=5)
Wilcoxon signed rank test: *, p<0.05; **, p<0.01
parameters | Uncoated (screw) | Coated (screw) | |
|---|---|---|---|
BIC (%) | Median | 22.5 | 69.0** |
SEM | 5.8 | 5.8 | |
(Min – Max) | (16.1 – 47.4) | (45.4 – 80.7) | |
Bone Ingrowth (%) | Median | 30.3 | 56.3** |
SEM | 2.0 | 3.4 | |
(Min – Max) | (23.9 – 33.9) | (41.9 – 59.4) |
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